Neuronal histological changes in fascia dentata from hippocampus after a permanent brain ischaemia in Gerbils.

Authors

  • Madelin Hérriman Estrada FACULTAD DE CIENCIAS MÉDICAS “CELIA SÁNCHEZ MANDULEY” MANZANILLO
  • Madeleyne González Broche FACULTAD DE CIENCIAS MÉDICAS “CELIA SÁNCHEZ MANDULEY” MANZANILLO
  • Carmen Ferrer Magadan FACULTAD DE CIENCIAS MÉDICAS “CELIA SÁNCHEZ MANDULEY” MANZANILLO
  • Misleidis Batista Vega FACULTAD DE CIENCIAS MÉDICAS “CELIA SÁNCHEZ MANDULEY” MANZANILLO
  • Sara Roblejo Córcoles FACULTAD DE CIENCIAS MÉDICAS “CELIA SÁNCHEZ MANDULEY” MANZANILLO

Keywords:

HIPPOCAMPUS/pathology, DENTATE GYRUS/pathology, GERBILLINAE, MODELS, ANIMAL

Abstract

It was made a comparative analytical study with the objective of describing behavior in time of neuronal damage dynamics after a permanent ischaemic trauma in neuron populations from hippocampus Fascia Dentate. There were selected 21 male Mongolia gerbils from 60 to 70 grams. It was planned taking three animals each time, which were anesthetized, and it was tied right intern carotid artery. There was sacrified three of them at 2, 4, 6, 12, 24, 48, and 72 hours after damage was produced, it was perfunded, and brain was extracted to process afterwards for getting stained histological plate with hematoxilin-eosin. It was made cell count by means of morphoestereologic technique. It was made a conventional division into two zones of Fascia dentate called FD1 and FD2. It was noticed the dynamics of morphological changes in time of neuron damage corresponding to pyknosis, Karyorrexis, and necrolisis as maximal expression of damage in zones of Fascia Dentate FD1 and FD2. In both areas studied, neurons damageless were observed from the beginning of study (2 hours) to 6 hours. Neurons in Karyorrexis presented a dynamic characterized by rough exaltation in numbers beginning from 4 hours and finishing at 48 hours, presence of a maximum top at 12 hours and a rough descention till 48 hours. Neurons in pyknosis presented a dynamic characterized by an increase in numbers appearing at two hours in FD1, and at four hours in FD2 to get a top between 12 and 24 hours, decreasing at 72 hours. Neurons in necrolisis appeared at 12 hours, increasing progressively in time in both areas to reach maximum values at 72 hours from trauma production. It were compared both study areas (FD1 and FD2), by means of statistics significance test, taking as significative a result corresponding to 95% (p < 0.05), that is a significant level of 5%. This way it was described the behavior in the time of ischemic damage prevailing in neuronal populations from the hippocampus Dentate Fascia. Finally we reach conclusions and recommendations in honor of perfectioning this study.

Downloads

Download data is not yet available.

References

1- Marston JH. The ufaw handbook on the care and managment of Laboratory Animals. 5 ed London: Churchill Livingston, 2000: 263 -74.

2- Boletín de la OMS. Nº. 234. Oficina OPS. Ciudad Habana, Cuba 2000.

3- Bonnekoh P.Selective vulnerability in the gerbil hippocampus: morphological changes alter 5 minute ischemia and long survival times. Acta Neuropathol. 1990; 80: 18-25.

4- Yamamoto J. Ultraestructural investigation of the CA region of the hippocampus after trasient cerebral ischemia in Gerbils. Acta Neuropathol. 2002; 80(5): 487.

5- Mickel K. Prominent white mather lesion develop in Mongolian gerbils treted with 100% normobaric oxygen after global brain ischemia. Acta Neuropathol. 2000; 70(5): 465.

6- Castro A, Foyo E, Perez U, Garcia J, Grijalva I. Comunicación arterial carotidobasilar en gerbos (Meriones unguiculatus). Rev. Neurol. 2001; 32: 225-228.

7- Yamada KA. New modelo f trasient hindbrain ischemia in Gerbils. J Neurosurg. 04;

60: 1054-8.

8- Norris ML, Adams CE.Suppression of aggresive behaviour in the Mongolian gerbiis (Merione unguiculatus). Lab Anim; (6): 695-9.

9- Sherardown MJ, Nielsen EO, Hansen AJ, Jacobsen P, Honore T. 2,3- dhydroxy-6-nitro-7-sulfamoyl-benzo(F) quinoxaline: a neuroprotectant for cerebral ischemia. Science. 2000; 247: 571-574.

10- Kirino T, Tamura A, Sanpo K. Selective vulnerability of the hippocampus to ischemia-

reversible and irreversible types of ischemic cell damage. Prog Brain Res 2005; 63: 39-58.

11- R. Truex;M.B. Carpenter Neuroantomia humana. La Habana: Edición revolucionaria-3 ed en español, tomada de la 5.ed inglesa. 1971.

12- E. Estrada; J. Perez. Neuroanatomía funcional 3 reimp.- (La habana): Ed revolucionaria, 1972-p. 324, 327, 241,342, 346-347.

13- R.H. Corrtach. W. Cowman Electron microscopic studies of the dentate gyrus of the rat F:Normal estructure with special reference to synaptic organization.-I. Comp. Neur 128: 359- 396. 1966.

Downloads

Published

2008-01-10

How to Cite

1.
Hérriman Estrada M, González Broche M, Ferrer Magadan C, Batista Vega M, Roblejo Córcoles S. Neuronal histological changes in fascia dentata from hippocampus after a permanent brain ischaemia in Gerbils. RM [Internet]. 2008 Jan. 10 [cited 2025 Jun. 1];12(1). Available from: https://revmultimed.sld.cu/index.php/mtm/article/view/1757

Issue

Vol. 12 No. 1 (2008)

Section

ARTÍCULOS ORIGINALES

License

Avisos de derechos de autor propuestos por Creative Commons

1. Política propuesta para revistas que ofrecen acceso abierto

Aquellos autores/as que tengan publicaciones con esta revista, aceptan los términos siguientes:
  1. Los autores/as conservarán sus derechos de autor y garantizarán a la revista el derecho de primera publicación de su obra, el cuál estará simultáneamente sujeto a la Licencia de reconocimiento de Creative Commons que permite a terceros compartir la obra siempre que se indique su autor y su primera publicación esta revista.
  2. Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
  3. Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos telemáticos institucionales o en su página web) antes y durante el proceso de envío, lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).